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KMID : 0032219910030020091
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Annals of Dermatology
1991 Volume.3 No. 2 p.91 ~ p.91
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Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA
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Park Kyang-Joon
Sung Ho-Suk
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Abstract
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In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.
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KEYWORD
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In situ hybridization, Condyloma acuminatum, Digoxigenin-11-dUTP, Radioactive isotope
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